Cowan E P, Nemo G J, Williams A E, Alexander R K, Vallejo A, Hewlett I K, Lal R B, Dezzutti C S, Gallahan D, George K, Pancake B A, Zucker-Franklin D, McCurdy P R, Tabor E
Transfusion Medicine Scientific Research Group, National Heart, Lung, and Blood Institute, Bethesda, Maryland, USA.
Transfusion. 1999 Aug;39(8):904-9. doi: 10.1046/j.1537-2995.1999.39080904.x.
It was reported recently that sequences corresponding to the human T-lymphotropic virus type I (HTLV-I) tax gene were detected in peripheral blood mononuclear cells from 8 to 11 percent of healthy blood donors without detectable antibodies to HTLV-I. A multicenter blind study was conducted to determine if these results could be independently confirmed.
Specimens were collected from 100 anti-HTLV-I-negative healthy blood donors and from 11 anti-HTLV-I- or anti-HTLV-II-positive individuals. All samples were coded and distributed to each of four independent testing laboratories for polymerase chain reaction analysis to detect sequences of the HTLV-I or HTLV-II tax gene, using detailed procedures specified by the laboratory reporting the original observation. Each laboratory also tested a dilution panel of a plasmid containing HTLV-I tax to determine the analytical sensitivity of the procedure.
The analytical sensitivity of the screening methods permitted detection of as few as 1 to 10 copies of the tax gene. However, HTLV-I tax sequences could not be detected in any of the anti-HTLV-I-negative blood donors at more than one test site.
HTLV-I tax sequences appear not to be present in this population of 100 blood donors negative for anti-HTLV-I.
最近有报道称，在8%至11%的健康献血者外周血单核细胞中检测到了与人类I型嗜T淋巴细胞病毒（HTLV-I）tax基因相对应的序列，这些献血者检测不到针对HTLV-I的抗体。开展了一项多中心盲法研究，以确定这些结果是否能得到独立验证。
从100名抗HTLV-I阴性的健康献血者以及11名抗HTLV-I或抗HTLV-II阳性个体中采集样本。所有样本均进行编码，并分发给四个独立检测实验室中的每一个，按照报告最初观察结果的实验室所指定的详细程序，通过聚合酶链反应分析来检测HTLV-I或HTLV-II tax基因序列。每个实验室还检测了含HTLV-I tax的质粒稀释样本组，以确定该检测程序的分析灵敏度。
筛查方法的分析灵敏度能够检测到低至1至10个拷贝的tax基因。然而，在超过一个检测位点的任何抗HTLV-I阴性献血者中均未检测到HTLV-I tax序列。
在这100名抗HTLV-I阴性的献血者群体中，似乎不存在HTLV-I tax序列。
