Diaz L A, Patel H, Calvanico N J
J Immunol. 1979 Feb;122(2):605-8.
This report concerns the isolation of bullous pemphigoid antigen from the nondialyzable urinary components of a patient with the disease. The isolation was accomplished by ion exchange chromatography and gel filtration. Pemphigoid antigen was found to be a basic glycoprotein that on SDS gel electrophoresis showed two major bands, one in the 18,000 m. w. region and the second with a m. w. of 74,000. Between these two bands, two additional bands appeared; one of 35,000 daltons and the other of 68,000 daltons. The 18,000 m. w. band was eluted from the gel and rerun on SDS gels. These gels showed the 18,000 m.w. band and also the appearance of the 35,000 and 74,000 m.w. bands. This finding indicates that urinary pemphigoid antigen may exist both as a single monomeric form and in polymeric aggregates.
本报告涉及从一名患有大疱性类天疱疮患者的非透析性尿液成分中分离大疱性类天疱疮抗原。分离通过离子交换色谱法和凝胶过滤法完成。发现类天疱疮抗原是一种碱性糖蛋白，在SDS凝胶电泳上显示出两条主要条带，一条在18,000分子量区域，另一条分子量为74,000。在这两条带之间，出现了另外两条带；一条为35,000道尔顿，另一条为68,000道尔顿。将18,000分子量的条带从凝胶上洗脱下来，并在SDS凝胶上重新运行。这些凝胶显示出18,000分子量的条带，以及35,000和74,000分子量条带的出现。这一发现表明尿液中的类天疱疮抗原可能以单一单体形式和聚合物聚集体形式存在。
