Diaz L A, Calvanico N J, Tomasi T B, Jordon R E
J Immunol. 1977 Feb;118(2):455-60.
A protein moiety from epidermal PBS-soluble products was isolated by gel filtration (Bio-Gel A-1.5m) and ion exchange chromatography (DEAE-cellulose). This protein (A-1-Epid) was not retarded by DEAE-cellulose in Tris-HCl buffer, 15mM, pH 8.1. By IEP against an antiserum to epidermal antigens, it showed a single cathodal arc. On disc electrophoresis, at low pH (4.3) a single band was apparent. On SDS gels this protein demonstrated two bands, one with a molecular weight of 20,000, and the second with a molecular weight of 9,200. This purified antigen was able to block the staining of the basement membrane zone produced by bullous pemphigoid antibodies on monkey esophagus and normal human skin with the use of indirect immunofluorescence. This study also demonstrates that bullous pemphigoid antigen (A-1-Epid) and a second epidermal protein (A-2-Epid) are present in the PBS-soluble products of human esophageal mucosa, saliva, and urine. These antigens appear to be unrelated with the blood group substances or secretor status of the donors.
通过凝胶过滤（Bio-Gel A-1.5m）和离子交换色谱法（DEAE-纤维素）从表皮PBS可溶性产物中分离出一种蛋白质部分。这种蛋白质（A-1-Epid）在15mM、pH 8.1的Tris-HCl缓冲液中不被DEAE-纤维素阻滞。通过针对表皮抗原抗血清的等电聚焦，它显示出一条单一的阴极弧。在圆盘电泳中，在低pH值（4.3）下出现一条单一的条带。在SDS凝胶上，这种蛋白质显示出两条带，一条分子量为20,000，另一条分子量为9,200。这种纯化的抗原能够通过间接免疫荧光阻断大疱性类天疱疮抗体在猴食管和正常人皮肤上产生的基底膜带染色。这项研究还表明，大疱性类天疱疮抗原（A-1-Epid）和第二种表皮蛋白质（A-2-Epid）存在于人类食管黏膜、唾液和尿液的PBS可溶性产物中。这些抗原似乎与供体的血型物质或分泌状态无关。
