干扰素-γ对I型干扰素受体的上调作用。

Upregulation of type I interferon receptor by IFN-gamma.

作者信息

Mizukoshi E, Kaneko S, Yanagi M, Ohno H, Matsushita E, Kobayashi K

机构信息

First Department of Internal Medicine, Kanazawa University School of Medicine, Ishikawa, Japan.

出版信息

J Interferon Cytokine Res. 1999 Sep;19(9):1019-23. doi: 10.1089/107999099313235.

DOI:10.1089/107999099313235

PMID:10505744

Abstract

Type I interferon (IFN) receptor has a multichain structure composed of at least two distinct subunits, IFNAR-1 and IFNAR-2. In the present study, we demonstrated that IFN-gamma induced the expression of mRNA for IFNAR-1 and IFNAR-2 in a human hepatoma cell line, HepG2 cells. The induction was dose and time dependent. Because of this result, we examined the effect of combined treatment with type I IFN and IFN-gamma. The intracellular 2-5A-synthetase activity induced by combined treatment was significantly higher than that by type I IFN alone. This study suggests that combined treatment with type I IFN and IFN-gamma may be more effective than that of type I IFN alone and that the upregulation of type I IFN receptor may be one of the reasons. Our findings may have some relevance to the clinical use of IFN.

摘要

I型干扰素（IFN）受体具有多链结构，由至少两个不同的亚基IFNAR-1和IFNAR-2组成。在本研究中，我们证明了IFN-γ可诱导人肝癌细胞系HepG2细胞中IFNAR-1和IFNAR-2的mRNA表达。这种诱导呈剂量和时间依赖性。基于这一结果，我们研究了I型干扰素与IFN-γ联合治疗的效果。联合治疗诱导的细胞内2-5A合成酶活性显著高于单独使用I型干扰素。本研究表明，I型干扰素与IFN-γ联合治疗可能比单独使用I型干扰素更有效，I型干扰素受体的上调可能是原因之一。我们的发现可能与干扰素的临床应用有一定关联。