Egea-Cortines M, Saedler H, Sommer H
Max-Planck-Institut für Züchtungsforschung, Carl-von-Linne Weg 10, 50829 Köln, Germany.
EMBO J. 1999 Oct 1;18(19):5370-9. doi: 10.1093/emboj/18.19.5370.
In Antirrhinum, floral meristems are established by meristem identity genes. Floral meristems give rise to floral organs in whorls, with their identity established by combinatorial activities of organ identity genes. Double mutants of the floral meristem identity gene SQUAMOSA and organ identity genes DEFICIENS or GLOBOSA produce flowers in which whorled patterning is partially lost. In yeast, SQUA, DEF and GLO proteins form ternary complexes via their C-termini, which in gel-shift assays show increased DNA binding to CArG motifs compared with DEF/GLO heterodimers or SQUA/SQUA homodimers. Formation of ternary complexes by plant MADS-box factors increases the complexity of their regulatory functions and might be the molecular basis for establishment of whorled phyllotaxis and combinatorial interactions of floral organ identity genes.
在金鱼草中，花分生组织由分生组织特征基因建立。花分生组织产生轮状的花器官，其特征由器官特征基因的组合活动确定。花分生组织特征基因SQUAMOSA与器官特征基因DEFICIENS或GLOBOSA的双突变体产生的花中，轮状模式部分丧失。在酵母中，SQUA、DEF和GLO蛋白通过其C末端形成三元复合物，在凝胶迁移试验中，与DEF/GLO异二聚体或SQUA/SQUA同二聚体相比，它们对CArG基序的DNA结合增加。植物MADS-box因子形成三元复合物增加了其调控功能的复杂性，可能是建立轮状叶序和花器官特征基因组合相互作用的分子基础。
