Tóth-Boconádi R, Szabó-Nagy A, Taneva S G, Keszthelyi L
Institute of Biophysics, Biological Research Centre of the Hungarian Academy of Sciences, H-6701, Szeged, Hungary.
FEBS Lett. 1999 Oct 1;459(1):5-8. doi: 10.1016/s0014-5793(99)01192-8.
Double flash experiments were performed in order to gain information about the characteristics of the N intermediates of the photocycle of bacteriorhodopsin. The N intermediates of wild-type bacteriorhodopsin and mutant T46V were excited at different delay times after the first laser flash which induced the photocycle and the electric responses were registered. These electric signals revealed that charge motions occurred in both cases, though charge translocation, i.e. H(+) pumping, could not be observed. The delay time dependence of the electric signals is characterized by two distinct processes corresponding to two substates of the N intermediates.
为了获取有关细菌视紫红质光循环中N中间体特性的信息，进行了双闪光实验。在诱导光循环的第一束激光闪光后的不同延迟时间，对野生型细菌视紫红质和突变体T46V的N中间体进行激发，并记录电响应。这些电信号表明，两种情况下均发生了电荷移动，尽管未观察到电荷转移，即H(+)泵浦。电信号的延迟时间依赖性由与N中间体的两个亚态相对应的两个不同过程表征。
