Institute of Biophysics, Biological Research Centre, Hungarian Academy of Sciences, Szeged, P. O. B. 521, H-6701, Hungary.
Bioelectrochemistry. 2011 Apr;81(1):17-21. doi: 10.1016/j.bioelechem.2010.12.003. Epub 2010 Dec 28.
All the intermediates of the bacteriorhodopsin photocycle are excitable with light of suitable wavelength. This property might regulate the activity in the cells when they are exposed in the nature to high light intensity. On the other hand this property is involved in many applications. In this study the ground state and M intermediate of dried oriented samples of wild-type bacteriorhodopsin and its mutant D96N were excited with 406 nm laser flashes. Substantial M populations were generated with quasi-continuous illumination. The decay of the absorption of M intermediate had three components: their lifetimes were very different for laser flash and quasi-continuous illuminations in cases of both bacteriorhodopsin species. The optical answer for the excitation of M intermediate had a lifetime of 2.2 ms. Electric signals for M excitation had large fast negative components and small positive components in the 100 μs time domain. The results are expected to have important implications for bioelectronic applications of bacteriorhodopsin.
细菌视紫红质光循环的所有中间体都可以被适当波长的光激发。当细胞在自然界中暴露于高强度光下时，这种特性可能会调节细胞的活性。另一方面，这种特性涉及到许多应用。在这项研究中，野生型细菌视紫红质及其突变体 D96N 的干燥定向样品的基态和 M 中间体分别用 406nm 激光闪光激发。用准连续照明产生了大量的 M 种群。M 中间体吸收的衰减有三个组成部分：对于激光闪光和准连续照明，两种细菌视紫红质的情况都有非常不同的寿命。M 中间体激发的光响应的寿命为 2.2ms。M 激发的电信号在 100μs 时域内有大的快速负分量和小的正分量。这些结果有望对细菌视紫红质在生物电子学中的应用有重要意义。
