Kuwae T, Shiota Y, Schmid P C, Krebsbach R, Schmid H H
Faculty of Pharmaceutical Sciences, Josai University, Keyakiday, Sakado, Saitama, Japan.
FEBS Lett. 1999 Oct 1;459(1):123-7. doi: 10.1016/s0014-5793(99)01226-0.
Polyunsaturated N-acylethanolamines (NAEs), including anandamide (20:4n-6 NAE), elicit a variety of biological effects through cannabinoid receptors, whereas saturated and monounsaturated NAEs are inactive. Arachidonic acid mobilization induced by treatment of intact mouse peritoneal macrophages with Ca2+ ionophore A23187 had no effect on the production of NAE or its precursor N-acylphosphatidylethanolamine (N-acyl PE). Addition of exogenous ethanolamine resulted in enhanced NAE synthesis by its N-acylation with endogenous fatty acids, but this pathway was not selective for arachidonic acid. Incorporation of (18)O from H2 (18)O-containing media into the amide carbonyls of both NAE and N-acyl PE demonstrated a rapid, constitutive turnover of both lipids.
多不饱和N-酰基乙醇胺（NAEs），包括花生四烯酸乙醇胺（20:4n-6 NAE），通过大麻素受体引发多种生物学效应，而饱和和单不饱和NAEs则无活性。用Ca2+离子载体A23187处理完整的小鼠腹腔巨噬细胞所诱导的花生四烯酸动员对NAE或其前体N-酰基磷脂酰乙醇胺（N-酰基PE）的产生没有影响。添加外源性乙醇胺通过其与内源性脂肪酸的N-酰化作用增强了NAE的合成，但该途径对花生四烯酸没有选择性。将含H2(18)O培养基中的(18)O掺入NAE和N-酰基PE的酰胺羰基中，证明了这两种脂质都有快速的、组成性的周转。
