Schmid P C, Krebsbach R J, Perry S R, Dettmer T M, Maasson J L, Schmid H H
Hormel Institute, University of Minnesota, Austin 55912, USA.
FEBS Lett. 1995 Nov 13;375(1-2):117-20. doi: 10.1016/0014-5793(95)01194-j.
Long-chain N-acylethanolamines (NAEs) were assayed in pig, sheep and cow brain by gas chromatography/mass spectrometry of their tert.-butyldimethylsilyl derivatives in the presence of deuterium-labeled internal standards. Immediately after death, total NAEs ranged from about 2.7 micrograms/g wet weight (sheep, cow) to 6.5 micrograms/g wet weight (pig) and consisted almost exclusively (99%) of saturated and monounsaturated species. Anandamide (20:4n-6 NAE) comprised about 1% of total NAE in pig and cow brain, but was absent in freshly extracted sheep brain. When pig brain was analysed after 0.5, 1, 3, 4 and 23 h at ambient temperature, NAE levels were found to increase substantially over the entire time period with 20:4n-6 NAE formation exhibiting a time lag compared to that of saturated and monounsaturated NAEs.
通过气相色谱/质谱法，在氘代内标存在的情况下，对叔丁基二甲基甲硅烷基衍生物进行分析，测定猪、羊和牛脑中的长链N-酰基乙醇胺（NAE）。死后立即测定，总NAE含量范围约为2.7微克/克湿重（羊、牛）至6.5微克/克湿重（猪），几乎全部（99%）由饱和及单不饱和种类组成。花生四烯酸乙醇胺（20:4n-6 NAE）在猪脑和牛脑中约占总NAE的1%，但在新鲜提取的羊脑中不存在。当在室温下对猪脑分别于0.5、1、3、4和23小时后进行分析时，发现NAE水平在整个时间段内大幅增加，与饱和及单不饱和NAE相比，20:4n-6 NAE的形成存在时间滞后。
