Flaks A, Flaks B
J Pathol. 1978 Oct;126(2):71-8. doi: 10.1002/path.1711260203.
Male inbred Leeds strain rats were given single intragastric doses of 1200 mg/kg of 3'-methyl-4-dimethylaminoazobenzene, autoprotective against liver necrosis and then sacrificed at intervals up to 14 months. About 40 per cent of the animals died, apparently as a result of pancreatic necrosis, between 9 and 13 days after treatment while the remainder survived until they were killed. The liver changes induced included an early intense ductular cell reaction, presistent hepatocellular abnormalities and at later stages, cholangiofibrosis and increasing numbers of parenchymal clear cells. Although no tumours arose as a result of treatment, the observed histopathological changes are similar to those seen during liver carcinogenesis induced by a variety of carcinogens and may be due specifically to the carcinogenic action of the azo dye.
给雄性近交系利兹大鼠单次灌胃剂量为1200毫克/千克的3'-甲基-4-二甲基氨基偶氮苯，其对肝坏死具有自身保护作用，然后在长达14个月的时间间隔内处死大鼠。约40%的动物在治疗后9至13天之间死亡，显然是胰腺坏死所致，而其余动物存活至被处死。诱导的肝脏变化包括早期强烈的小胆管细胞反应、持续的肝细胞异常，以及后期的胆管纤维化和实质透明细胞数量增加。虽然治疗未导致肿瘤发生，但观察到的组织病理学变化与多种致癌物诱导的肝癌发生过程中所见的变化相似，可能具体归因于偶氮染料的致癌作用。
