采用抑制化学发光检测的高效液相色谱法测定多酚类物质。
Determination of polyphenols by high-performance liquid chromatography with inhibited chemiluminescence detection.
作者信息
Cui H, He C, Zhao G
机构信息
Department of Chemistry, University of Science and Technology of China, Hefei, Anhui, P.R. China.
出版信息
J Chromatogr A. 1999 Sep 3;855(1):171-9. doi: 10.1016/s0021-9673(99)00670-6.
A chemiluminescence reaction detector was developed for the detection of polyphenols separated by HPLC based on the inhibition of chemiluminescence from the luminol-potassium hexacyanoferrate(III) reaction by polyphenols. The separation was carried out on a RP-C18 column at 37 degrees C by using stepwise gradient elutions. The detection limits are in the range of 6.8 x 10(-7)-2.0 x 10(-9) g/ml for catechol, protocatechuic acid, chlorogenic acid, rutin, resorcinol, hydroquinone and p-tert.butylpyrocatechol. The method is sensitive, selective, fast and simple. It has been successfully applied to the determination of chlorogenic acid and rutin in real tobacco samples.
基于多酚对鲁米诺-铁氰化钾（III）反应化学发光的抑制作用，开发了一种化学发光反应检测器，用于检测经高效液相色谱分离的多酚。分离在RP-C18柱上于37℃进行，采用梯度洗脱。儿茶酚、原儿茶酸、绿原酸、芦丁、间苯二酚、对苯二酚和对叔丁基邻苯二酚的检测限在6.8×10⁻⁷ - 2.0×10⁻⁹ g/ml范围内。该方法灵敏、选择性好、快速且简便。已成功应用于实际烟草样品中绿原酸和芦丁的测定。
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