高效液相色谱法测定山里红叶片中8种多酚类物质的含量

HPLC determination of eight polyphenols in the leaves of Crataegus pinnatifida Bge. var. major.

作者信息

Ying Xixiang, Wang Rongxiang, Xu Jing, Zhang Wenjie, Li Haibo, Zhang Chaoshen, Li Famei

机构信息

School of Pharmacy, Liaoning University of TCM, 79 Chongshan East Road, Shenyang, Liaoning Province, PR China.

出版信息

J Chromatogr Sci. 2009 Mar;47(3):201-5. doi: 10.1093/chromsci/47.3.201.

DOI:10.1093/chromsci/47.3.201

PMID:19298706

Abstract

A simple high-performance liquid chromatographic (HPLC) assay using the internal standard method is developed for the simultaneous determination of eight polyphenols. The analyzed compounds isolated from the leaves of Crataegus pinnatifida Bge. var. major include chlorogenic acid, vitexin-4"-O-glucoside, vitexin-2"-O-rhamnoside, vitexin, rutin, hyperoside, isoquercitrin, and quercetin. HPLC analysis is performed on a Diamonsil C18 analytical column (150 x 4.6 mm, i.d., 5-microm) using solvent (A) acetonitrile-tetrahydrofuran (95:5, v/v) and (B) 1% aqueous phosphoric acid as the mobile phase with UV absorption at 270 nm. The calibration curves of the eight polyphenols are linear (r(2) > 0.9992) over the concentration range of 0.0894-120.0 microg/mL. The mean recoveries are 95.4% to 98.1%. The results indicate that the HPLC method developed can easily be applied to the determination of eight polyphenols in the leaves of Crataegus pinnatifida Bge. var. major.

摘要

建立了一种采用内标法的简单高效液相色谱（HPLC）测定方法，用于同时测定8种多酚类物质。从山里红叶片中分离得到的分析化合物包括绿原酸、牡荆素-4”-O-葡萄糖苷、牡荆素-2”-O-鼠李糖苷、牡荆素、芦丁、金丝桃苷、异槲皮苷和槲皮素。HPLC分析在Diamonsil C18分析柱（150×4.6 mm，内径，5μm）上进行，使用溶剂（A）乙腈-四氢呋喃（95:5，v/v）和（B）1%磷酸水溶液作为流动相，在270 nm处进行紫外吸收检测。8种多酚类物质的校准曲线在0.0894 - 120.0μg/mL浓度范围内呈线性（r(2)>0.9992）。平均回收率为95.4%至98.1%。结果表明，所建立的HPLC方法可轻松应用于山里红叶片中8种多酚类物质的测定。