Fodor E, Devenish L, Engelhardt O G, Palese P, Brownlee G G, García-Sastre A
Sir William Dunn School of Pathology, University of Oxford, Oxford OX1 3RE, United Kingdom.
J Virol. 1999 Nov;73(11):9679-82. doi: 10.1128/JVI.73.11.9679-9682.1999.
We have rescued influenza A virus by transfection of 12 plasmids into Vero cells. The eight individual negative-sense genomic viral RNAs were transcribed from plasmids containing human RNA polymerase I promoter and hepatitis delta virus ribozyme sequences. The three influenza virus polymerase proteins and the nucleoprotein were expressed from protein expression plasmids. This plasmid-based reverse genetics technique facilitates the generation of recombinant influenza viruses containing specific mutations in their genes.
我们通过将12个质粒转染到Vero细胞中拯救出了甲型流感病毒。8个单独的负义基因组病毒RNA是从含有人类RNA聚合酶I启动子和丁型肝炎病毒核酶序列的质粒转录而来的。三种流感病毒聚合酶蛋白和核蛋白是从蛋白质表达质粒中表达出来的。这种基于质粒的反向遗传学技术有助于产生在其基因中含有特定突变的重组流感病毒。