雄激素非依赖性hoxb-13在小鼠前列腺中的表达
Androgen-independent expression of hoxb-13 in the mouse prostate.
作者信息
Sreenath T, Orosz A, Fujita K, Bieberich C J
机构信息
Functional Genomics Unit, NIDCR, National Institutes of Health, Bethesda, Maryland, USA.
出版信息
Prostate. 1999 Nov 1;41(3):203-7. doi: 10.1002/(sici)1097-0045(19991101)41:3<203::aid-pros8>3.0.co;2-j.
BACKGROUND
Hox genes encode transcriptional regulatory proteins that are largely responsible for establishing the body plan of all metazoan organisms. A subset of Hox genes is expressed during the period of organogenesis and into adulthood. hoxb-13 is a recently-described member of the Hox gene family that is expressed in the spinal cord, hindgut, and urogenital sinus during embryogenesis.
METHODS
Northern blot and in situ hybridization analyses of hoxb-13 expression in adult mouse tissues were performed.
RESULTS
hoxb-13 mRNA is restricted to the prostate gland and distal colon in adult animals. In situ hybridization of mouse prostate tissue demonstrated that hoxb-13 is expressed in the epithelial cells of the ventral, dorsal, lateral, and anterior prostate lobes. Accumulation of hoxb-13 mRNA is not diminished following castration.
CONCLUSIONS
These data demonstrate that hoxb-13 expression is androgen-independent in mouse prostate glands. The identification of hoxb-13 as an androgen-independent gene expressed in adult mouse prostate epithelial cells provides a new potential target for developing therapeutics to treat advanced prostate cancer.
背景
Hox基因编码转录调节蛋白，在很大程度上负责建立所有后生动物的身体结构。一部分Hox基因在器官发生期及成年期表达。hoxb - 13是Hox基因家族中最近描述的一个成员，在胚胎发育过程中在脊髓、后肠和泌尿生殖窦中表达。
方法
对成年小鼠组织中hoxb - 13的表达进行Northern印迹和原位杂交分析。
结果
在成年动物中，hoxb - 13 mRNA局限于前列腺和远端结肠。小鼠前列腺组织的原位杂交显示，hoxb - 13在腹侧、背侧、外侧和前叶前列腺的上皮细胞中表达。去势后hoxb - 13 mRNA的积累并未减少。
结论
这些数据表明，hoxb - 13在小鼠前列腺中的表达不依赖雄激素。hoxb - 13作为在成年小鼠前列腺上皮细胞中表达的不依赖雄激素的基因，为开发治疗晚期前列腺癌的疗法提供了一个新的潜在靶点。
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