聚乙二醇可增强脂质体与细胞的结合及脂质体转染。

Polyethylene glycol enhances lipoplex-cell association and lipofection.

作者信息

Ross P C, Hui S W

机构信息

Membrane Biophysics Laboratory, Molecular and Cellular Biophysics Department, Roswell Park Cancer Institute, Elm and Carlton Streets, Buffalo, NY, USA.

出版信息

Biochim Biophys Acta. 1999 Oct 15;1421(2):273-83. doi: 10.1016/s0005-2736(99)00132-7.

DOI:10.1016/s0005-2736(99)00132-7

PMID:10518697

Abstract

The association between liposome-DNA complexes (lipoplexes) and targeted cell membranes is a limiting step of cationic liposome-mediated transfection. A novel technique was developed where lipoplex-cell membrane association is enhanced by the addition of 2-6% polyethylene glycol (PEG) to the transfection media. Lipoplex-cell association was found to increase up to 100 times in the presence of PEG. Transfection increased correspondingly in the presence of PEG. This increase was found in several cell lines. These results show that lipoplex adsorption to cell membranes is a critical step in liposome-mediated transfection. This step can be facilitated by PEG-induced particle aggregation.

摘要

脂质体 - DNA复合物（脂质复合物）与靶向细胞膜之间的结合是阳离子脂质体介导转染的一个限制步骤。开发了一种新技术，通过向转染培养基中添加2 - 6%的聚乙二醇（PEG）来增强脂质复合物与细胞膜的结合。发现在PEG存在下，脂质复合物与细胞的结合增加了多达100倍。在PEG存在下，转染相应增加。在几种细胞系中都发现了这种增加。这些结果表明，脂质复合物吸附到细胞膜上是脂质体介导转染的关键步骤。PEG诱导的颗粒聚集可以促进这一步骤。