Schmid E, Braselmann H, Bauchinger M
GSF, National Research Center for Environment and Health, Institute of Radiobiology, 85764 Neuherberg, Germany.
Radiat Res. 1999 Nov;152(5):563-6.
The debate on the validity of the ratios of radiation-induced yields of chromosome aberrations, in particular the F value (dicentrics/ring chromosomes), as a chromosomal fingerprint of radiation quality is still in progress. From a recent analysis of their experimental data, Sasaki et al. (Radiat. Res. 150, 253-258, 1998) noted that despite a considerable variability in the data, the limiting F value at the lowest doses, or the F(0) value, obviously decreased with increasing LET, indicating that the LET could be a factor that determines the F value. We have reassessed here our own 13 cytogenetic data sets that cover a range of dose-averaged LET of 0.5 to 150 keV/microm in terms of this F(0)-value approach, but we could not confirm such a dependence on LET at very low doses. The validity of the F value as a biomarker therefore remains questionable. For a final evaluation, scoring of a far greater number of cells at low doses would be necessary to reduce the large error ranges of F values.
关于辐射诱发染色体畸变产率的比率，尤其是F值（双着丝粒染色体/环状染色体）作为辐射品质的染色体指纹的有效性的争论仍在进行中。从最近对他们实验数据的分析来看，佐佐木等人（《辐射研究》150, 253 - 258, 1998年）指出，尽管数据存在相当大的变异性，但最低剂量下的极限F值，即F(0)值，显然随着传能线密度（LET）的增加而降低，这表明LET可能是决定F值的一个因素。在此，我们根据这种F(0)值方法重新评估了我们自己的13个细胞遗传学数据集，这些数据集涵盖了0.5至150 keV/μm的一系列剂量平均LET，但我们无法证实在极低剂量下存在这种对LET的依赖性。因此，F值作为生物标志物的有效性仍然值得怀疑。为了进行最终评估，有必要在低剂量下对大量细胞进行计数，以减少F值的巨大误差范围。
