基于由稳定和不稳定染色体畸变计算得出的F比率，对密集电离辐射的染色体指纹进行的理论和实验测试。

Theoretical and experimental tests of a chromosomal fingerprint for densely ionizing radiation based on F ratios calculated from stable and unstable chromosome aberrations.

作者信息

Lucas J N, Deng W, Oram S W, Hill F S, Durante M, George K, Wu H, Owens C L, Yang T

机构信息

University of California, Lawrence Livermore National Laboratory, Livermore 94550, USA.

出版信息

Radiat Res. 1999 Jan;151(1):85-91.

PMID:9973089

Abstract

In the present study, F ratios for both stable chromosome aberrations, i.e. ratios of translocations to pericentric inversions, and unstable aberrations, i.e. dicentrics and centric rings, were measured using fluorescence in situ hybridization. F ratios for stable aberrations measured after exposure to low (2.89 Gy 60Co gamma rays) and high-LET (0.25 Gy 56Fe ions; 1.25 Gy 56Fe ions; 3.0 Gy 12C ions) radiation were 6.5 +/- 1.5, 4.7 +/- 1.6, 9.3 +/- 2.5 and 10.4 +/- 3.0, respectively. F ratios for unstable aberrations measured after low (2.89 Gy 60Co gamma rays) and high-LET (0.25 Gy 56Fe ions; 3.0 Gy 12C ions) radiations were 6.5 +/- 1.6, 6.3 +/- 2.3 and 11.1 +/- 3.7, respectively. No significant difference between the F ratios for low- and high-LET radiation was found. Further tests on the models for calculation of the F ratio proposed by Brenner and Sachs (Radiat. Res. 140, 134-142, 1994) showed that the F ratio may not be straightforward as a practical fingerprint for densely ionizing radiation.

摘要

在本研究中，使用荧光原位杂交技术测量了稳定染色体畸变（即易位与臂间倒位的比率）和不稳定畸变（即双着丝粒和着丝粒环）的F比率。在受到低剂量（2.89 Gy 60Coγ射线）和高传能线密度（0.25 Gy 56Fe离子；1.25 Gy 56Fe离子；3.0 Gy 12C离子）辐射后测量的稳定畸变的F比率分别为6.5±1.5、4.7±1.6、9.3±2.5和10.4±3.0。在受到低剂量（2.89 Gy 60Coγ射线）和高传能线密度（0.25 Gy 56Fe离子；3.0 Gy 12C离子）辐射后测量的不稳定畸变的F比率分别为6.5±1.6、6.3±2.3和11.1±3.7。未发现低传能线密度和高传能线密度辐射的F比率之间存在显著差异。对Brenner和Sachs（Radiat. Res. 140, 134 - 142, 1994）提出的F比率计算模型进行的进一步测试表明，F比率作为致密电离辐射的实际特征标记可能并非那么简单直接。