Sasaki F, Kano R, Nakamura Y, Tsujimoto H, Yamamoto S, Hasegawa A
Department of Pathobiology, Nihon University School of Veterinary Medicine.
Microbiol Res. 1999 Sep;154(2):167-72. doi: 10.1016/S0944-5013(99)80010-X.
The 23S ribosomal DNAs were amplified from 11 strains of Leptospira interrogans sensu lato by polymerase chain reaction (PCR) and sequenced. The PCR products of about 290-bp DNA fragments indicated more than 97% sequence similarity to each other. The phylogenetic tree based on the 23S ribosomal DNAs obtained in this study revealed that 11 strains of L. interrogans examined composed a cluster distinct to that of L. weilii and L. borgpetersenii, confirming that these strains were similar to strain Moulton of L. interrogans serovar canicola in 23S rDNA sequence.
