从临床样本中直接对钩端螺旋体种进行系统发育鉴定的新分子靶标：16S rRNA 的替代基因。
New molecular target for the phylogenetic identification of Leptospira species directly from clinical samples: an alternative gene to 16S rRNA.
机构信息
Basic Science Research Group, Graduates School - CES University. Medellín, Antioquia, Colombia.
Microbiology School, Primary Immunodeficiencies Group, University of Antioquia. Medellín, Antioquia, Colombia.
出版信息
Rev Soc Bras Med Trop. 2020 Mar 16;53:e20190333. doi: 10.1590/0037-8682-0333-2019. eCollection 2020.
INTRODUCTION
Phylogenetic analysis of the 16S ribosomal gene initial region is used to identify Leptospira isolates at the species level from clinical samples. Unfortunately, this method cannot differentiate between some intermediates and saprophytic species.
METHODS
We used comparative genomic analysis between 35 Leptospira species to find new molecular targets for Leptospira species identification.
RESULTS
We proposed the use of the rpoC gene, encoding the DNA-directed RNA polymerase β-subunit, for identifying 35 Leptospira species.
CONCLUSIONS
The rpoC gene can be a molecular target to identify the main species of the Leptospira genus directly from clinical samples.
简介
16S 核糖体基因初始区域的系统发育分析用于从临床样本中鉴定种水平的钩端螺旋体分离株。不幸的是，这种方法不能区分一些中间种和腐生种。
方法
我们使用 35 种钩端螺旋体之间的比较基因组分析来寻找用于钩端螺旋体种鉴定的新分子靶标。
结果
我们提出使用 rpoC 基因，该基因编码 DNA 指导的 RNA 聚合酶β亚基，用于鉴定 35 种钩端螺旋体。
结论
rpoC 基因可以成为从临床样本中直接鉴定钩端螺旋体属主要种的分子靶标。
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