Anai T, Kono N, Kosemura S, Yamamura S, Hasegawa K
Institute of Applied Biochemistry, University of Tsukuba, Ibaraki, Japan.
DNA Seq. 1998;9(5-6):329-33.
A gene homologue to the auxin-inducible SAUR (Small Auxin Up RNAs) of soybean was isolated from a cDNA and a genomic library of radish. The cDNA clone was about 470 bp in length and contained a DST (Down STream) element that was involved in mRNA instability in the 3'-untranslated region. The genomic clone contained two short sequences that related to auxin-responsible core sequences (AGTCTC and TATCCCAC) in its promoter region. The radish SAUR transcript was accumulated by auxin and cycloheximide treatments in the same way as that in other plant species. However, the NDE and DUE elements that were reported in all of the other SAUR gene promoters were not conserved in this radish SAUR gene. The results suggest that the promoter activity of the SAUR gene will be regulated by only two short sequences.
从萝卜的cDNA文库和基因组文库中分离出了一个与大豆生长素诱导型SAUR（小生长素上调RNA）基因同源的基因。该cDNA克隆长度约为470 bp，在其3'非翻译区含有一个与mRNA不稳定性有关的DST（下游）元件。基因组克隆在其启动子区域含有两个与生长素响应核心序列（AGTCTC和TATCCCAC）相关的短序列。萝卜SAUR转录本通过生长素和环己酰亚胺处理的积累方式与其他植物物种相同。然而，在所有其他SAUR基因启动子中报道的NDE和DUE元件在该萝卜SAUR基因中并不保守。结果表明，SAUR基因的启动子活性仅由两个短序列调控。
