Oeller P W, Keller J A, Parks J E, Silbert J E, Theologis A
Plant Gene Expression Center, Albany, CA 94710.
J Mol Biol. 1993 Oct 20;233(4):789-98. doi: 10.1006/jmbi.1993.1555.
Two early auxin-inducible genes (PS-IAA4/5 and PSIAA6) from pea were cloned using previously isolated complementary DNA sequences. They are present in single copy per haploid genome, and are members of a large divergent multigene family that encodes similar proteins. The genes were structurally characterized and sequence analysis of their 5'-flanking regions revealed the presence of several highly conserved sequences found in various auxin-regulated genes from other plant species. Their coding regions are interrupted by three and two introns, respectively. Introns two and three of PS-IAA4/5 and introns one and two of PS-IAA6 are located in identical positions. These genes encode proteins of 189 (21,036 Da) and 179 (20,330 Da) residues that are 46% identical. They also share a significant degree of identity (42 to 80%) with other proteins encoded by auxin regulated genes in soybean, mungbean and Arabidopsis thaliana. All proteins contain four conserved domains ranging in size from 9 to 43 amino acids. Their most prominent feature is the presence of a highly charged N terminus consisting of two clusters of acidic residues separated by a cluster of basic amino acids.
利用先前分离的互补DNA序列克隆了豌豆的两个早期生长素诱导基因（PS-IAA4/5和PS-IAA6）。它们在单倍体基因组中以单拷贝形式存在，是一个编码相似蛋白质的大型多基因家族的成员。对这些基因进行了结构表征，对其5'侧翼区域的序列分析揭示了在其他植物物种的各种生长素调节基因中发现的几个高度保守的序列。它们的编码区分别被三个和两个内含子打断。PS-IAA4/5的第二和第三个内含子以及PS-IAA6的第一和第二个内含子位于相同位置。这些基因分别编码189个（21,036道尔顿）和179个（20,330道尔顿）残基的蛋白质，它们的同源性为46%。它们与大豆、绿豆和拟南芥中由生长素调节基因编码的其他蛋白质也有显著程度的同源性（42%至80%）。所有蛋白质都含有四个大小从9到43个氨基酸不等的保守结构域。它们最显著的特征是存在一个高度带电的N端，由两簇酸性残基组成，中间隔着一簇碱性氨基酸。
