Malícková K, Sedláková Z, Rerichová M, Marecková H, Fucíková T
Ustav klinické imunologie a alergologie, Vseobecná fakultní nemocnice a 1. lékarská fakulta Univerzity Karlovy, Praha.
Epidemiol Mikrobiol Imunol. 1999 Aug;48(3):106-10.
The phagocytic activity was determined in human polymorphonuclear leucocytes using the flow cytometry technique with microspheric hydrophilic particles labelled with fluorescein isothiocyanate. Non-specific adherence of particles is discussed and normal values of phagocytic activity are presented. The advantages of this method include simplified sample preparation using whole blood, rapidity of the test, precise phagocytosis quantification and possibility of archiving all findings in a computer.
采用流式细胞术,利用异硫氰酸荧光素标记的微球亲水性颗粒,测定人多形核白细胞的吞噬活性。讨论了颗粒的非特异性黏附情况,并给出了吞噬活性的正常值。该方法的优点包括使用全血简化样本制备、检测快速、吞噬作用定量精确以及能够将所有结果存档于计算机。