Glass W, Jenssen H L, Mix E, Friedrich A
Biomed Biochim Acta. 1984;43(2):187-96.
A flow cytometric technique is described for measurement of phagocytic activity in human leucocytes using FITC-labelled latex particles (0.77 micron). Cell suspensions separated from peripheral blood by dextran sedimentation were hemolyzed to avoid particle uptake by erythrocytes. The leucocytes were incubated for various time intervals with a standardized solution of 2 x 10(7) FITC-labelled latex particles per 2 x 10(5) leucocytes in 0.6 ml medium. Relative fluorescence per cell was measured on the basis of 10(5) cells, and a simple technique has been described to evaluate data characteristic for different steps of phagocytosis and to discriminate between phagocytizing and non-phagocytizing cells. In comparison, rice starch phagocytosis has been measured microscopically. A positive correlation between both methods has been found in 11 patients with different disease-related phagocytic capacity.
描述了一种流式细胞术，用于使用异硫氰酸荧光素（FITC）标记的乳胶颗粒（0.77微米）测量人白细胞的吞噬活性。通过葡聚糖沉降从外周血中分离出的细胞悬液进行溶血，以避免红细胞摄取颗粒。将白细胞与每2×10⁵个白细胞含有2×10⁷个FITC标记乳胶颗粒的标准化溶液在0.6毫升培养基中孵育不同时间间隔。基于10⁵个细胞测量每个细胞的相对荧光，并描述了一种简单的技术来评估吞噬作用不同步骤的数据特征，并区分吞噬细胞和非吞噬细胞。相比之下，已通过显微镜测量了大米淀粉吞噬作用。在11名具有不同疾病相关吞噬能力的患者中发现了两种方法之间的正相关。
