Singal D P, Li J, Lei K
Department of Pathology and Molecular Medicine, McMaster University, Hamilton, Ont, Canada.
Immunol Lett. 1999 Sep 1;69(3):301-6. doi: 10.1016/s0165-2478(99)00108-x.
We analyzed HLA-DR antigens and microsatellite Bat2 alleles in 97 adult caucasian patients with classical seropositive rheumatoid arthritis (RA) and 95 normal healthy controls. The results demonstrate that the prevalence of microsatellite Bat2 138 allele was significantly higher in RA-susceptibility DRB1 QKRAA/QRRAA epitope-negative patients as compared with normal controls. Analysis of the data suggested that Bat2 138 allele has primary association with RA-susceptibility in QKRAA/QRRAA epitope-negative patients. The Bat2 138 allele thus provides an additional risk in RA-susceptibility. In addition, microsatellite Bat2 138 allele showed a highly significant positive association with microsatellite D6S273 138 allele, which has similar (identical) association with RA development in DRB1 QKRAA/QRRAA epitope-negative patients. The present data demonstrate that DRB1 QKRAA/QRRAA epitope and microsatellite Bat2 138/D6S273 138 alleles more completely define the risk for development of RA. The results in the present study therefore suggest that two regions in MHC, class II (DRB1) and class III (Bat2 and D6S273 in HSP70-Bat2 region), contribute to susceptibility to RA.
我们分析了97例经典血清阳性类风湿性关节炎（RA）成年白种患者及95例正常健康对照者的HLA-DR抗原和微卫星Bat2等位基因。结果显示，与正常对照相比，微卫星Bat2 138等位基因在RA易感性DRB1 QKRAA/QRRAA表位阴性患者中的患病率显著更高。数据分析表明，在QKRAA/QRRAA表位阴性患者中，Bat2 138等位基因与RA易感性存在主要关联。因此，Bat2 138等位基因在RA易感性方面增加了额外风险。此外，微卫星Bat2 138等位基因与微卫星D6S273 138等位基因显示出高度显著的正相关，在DRB1 QKRAA/QRRAA表位阴性患者中，D6S273 138等位基因与RA发展存在相似（相同）的关联。目前的数据表明，DRB1 QKRAA/QRRAA表位以及微卫星Bat2 138/D6S273 138等位基因能更全面地界定RA发生的风险。因此，本研究结果提示，MHC中的两个区域，即II类（DRB1）和III类（HSP70-Bat2区域中的Bat2和D6S273），与RA易感性有关。
