Perfumi M, Ciccocioppo R, Angeletti S, Cucculelli M, Massi M
Department of Pharmacological Sciences and Experimental Medicine, University of Camerino, Italy.
Alcohol Alcohol. 1999 Sep-Oct;34(5):690-8. doi: 10.1093/alcalc/34.5.690.
The present study investigated the effect of acute intragastric (i.g.) administration of dry Hypericum perforatum extract (HPE), containing 0.3% hypericin, on ethanol intake in genetically selected Marchigian Sardinian alcohol-preferring (msP) rats. The i.g. administration of HPE, 125 or 250 mg/kg, induced a 30-40% reduction in ethanol intake in rats offered 10% (v/v) ethanol for 2 h/day. The effect of these doses was selective, since they modified neither food intake nor food-associated drinking; neither did the same doses modify the rat's gross behaviour in the open-field test. A dose of 500 mg/kg frequently induced immobility and a general suppression of ingestive behaviour. In rats offered 10% ethanol for 12 h/day, ethanol intake following treatment with 250 mg/kg HPE was significantly lower than that of controls for up to 10 h. The effect on ethanol intake was not related to the antidepressant-like effect of HPE revealed in the forced swimming test. In this regard, the effect on ethanol intake was observed after a single administration of 125 mg/kg, whereas the antidepressant effect was observed only after repeated treatment with doses higher than 125 mg/kg HPE. The i.g. administration of HPE, 250 mg/kg, did not affect blood-alcohol levels following i.g. treatment with 0.7 g/kg ethanol, the amount usually ingested in a single drinking episode; thus, the effect is not related to changes in the pharmacokinetics of ethanol. The present study shows that HPE markedly reduces ethanol intake in msP rats, without significantly modifying food intake.
本研究调查了急性胃内（i.g.）给予含0.3%金丝桃素的干燥贯叶连翘提取物（HPE）对基因选择的马尔凯大区撒丁岛嗜酒（msP）大鼠乙醇摄入量的影响。以125或250mg/kg的剂量胃内给予HPE，可使每天提供10%（v/v）乙醇、持续2小时的大鼠的乙醇摄入量降低30 - 40%。这些剂量的作用具有选择性，因为它们既不改变食物摄入量，也不改变与食物相关的饮水；相同剂量也不会改变大鼠在旷场试验中的总体行为。500mg/kg的剂量经常会导致大鼠不动，并普遍抑制摄食行为。对于每天提供10%乙醇、持续12小时的大鼠，用250mg/kg HPE处理后，在长达10小时内乙醇摄入量显著低于对照组。对乙醇摄入量的影响与强迫游泳试验中显示的HPE的抗抑郁样作用无关。在这方面，单次给予125mg/kg后观察到对乙醇摄入量的影响，而抗抑郁作用仅在重复给予高于125mg/kg HPE的剂量后才观察到。以250mg/kg的剂量胃内给予HPE，在用0.7g/kg乙醇（单次饮酒通常摄入的量）进行胃内处理后，不影响血液酒精水平；因此，该作用与乙醇药代动力学的变化无关。本研究表明，HPE可显著降低msP大鼠的乙醇摄入量，而不会显著改变食物摄入量。
