Aaboe M, Pinholt E M, Schou S, Hjørting-Hansen E
Department of Oral Surgery, School of Dentistry, Faculty of Health Sciences, University of Copenhagen, Denmark.
Clin Oral Implants Res. 1998 Jun;9(3):163-9. doi: 10.1034/j.1600-0501.1998.090303.x.
The present study describes the use of unbiased stereological methods for the quantitative evaluation of the amount of regenerated bone. Using the principle of guided bone regeneration the amount of regenerated bone after placement of degradable or non-degradable membranes covering defects in rabbit calvaria was compared. Forty rabbits were divided into 5 groups. A titanium microplate was placed over the defect to prevent collapse of the membrane. The non-degradable expanded polytetrafluoroethylene membrane and the degradable Polyglactin 910 material were both placed unicortically and bicortically. Undecalcified sections were prepared for stereologic evaluation after an observation period of 8 weeks. Complete bone healing of the defects was not observed in any of the specimens. Unbiased stereologic estimates revealed 48% bone regeneration in defects covered by 2 ePTFE membranes, and 12% in defects covered by 2 Polyglactin 910 membranes. Defects covered by 1 ePTFE or 1 Polyglactin 910 membranes revealed 10% or 18% bone regeneration, respectively. The control group regenerated 14%. The major difference of the estimates was caused by real difference between specimens, i.e. biologic variation, whereas only minimal variance was added by the stereologic estimation procedure.
本研究描述了使用无偏倚的体视学方法对再生骨量进行定量评估。利用引导骨再生原理，比较了在兔颅骨缺损处放置可降解或不可降解膜后再生骨的量。40只兔子被分为5组。在缺损上方放置一块钛微型板以防止膜塌陷。不可降解的膨体聚四氟乙烯膜和可降解的聚乙交酯910材料均以单皮质和双皮质方式放置。在观察期8周后制备不脱钙切片进行体视学评估。在任何标本中均未观察到缺损的完全骨愈合。无偏倚的体视学估计显示，由2层膨体聚四氟乙烯膜覆盖的缺损中有48%的骨再生，由2层聚乙交酯910膜覆盖的缺损中有12%的骨再生。由1层膨体聚四氟乙烯膜或1层聚乙交酯910膜覆盖的缺损分别显示有10%或18%的骨再生。对照组的骨再生率为14%。估计值的主要差异是由标本之间的实际差异即生物学变异引起的，而体视学估计程序仅增加了最小的方差。
