Mustafa K, Silva Lopez B, Hultenby K, Wennerberg A, Arvidson K
Department of Prosthetic Dentistry, Faculty of Odontology, Karolinska Institutet, Stockholm, Sweden.
Clin Oral Implants Res. 1998 Jun;9(3):195-207. doi: 10.1034/j.1600-0501.1998.090307.x.
The purpose of this study was to determine the effect of c.p. titanium surfaces blasted with TiO2 particles on the biological responses of human gingival fibroblasts (HGF). Fibroblast morphology and attachment were investigated on turned (control) titanium surfaces and those blasted with 45 microns (standard), 45-63 microns, and 63-90 microns TiO2 particles. The specimens were analyzed using a confocal laser scanner and SEM. The cell profile areas were measured using a semiautomatic interactive image analyser. The figures were expressed as percent of attachment. The turned samples had the smoothest surfaces and the roughest were those blasted with 63-90 microns. All TiO2 blasted specimens had homogeneous surfaces. Cells appeared to flatten, spread and form cellular bridges with the adjacent cells. Fibroblasts on the turned titanium surfaces appeared to follow the direction of the fine irregularities on the surface but tended to spread haphazardly on the blasted surfaces. The attachment assays showed no significant difference in the percentage of fibroblast cell attachment on the standard surfaces compared to the turned surfaces. Both surfaces blasted with 45-63 microns or 63-90 microns had significantly (P < 0.05) lower percentages of cell attachment than the control. The surfaces blasted with 63-90 microns particles had the lowest rate of cell attachment. A significant correlation (P < 0.01) was found between the degree of particle size and attachment of fibroblasts after 1-72 h. It is concluded that surface micro-texture influences the attachment and growth of HGF: surfaces blasted with 45 microns TiO2 do not inhibit fibroblast attachment and smooth or finely grooved surfaces could be conducive to cellular attachment.
本研究的目的是确定用二氧化钛颗粒喷砂处理的商业纯钛表面对人牙龈成纤维细胞（HGF）生物学反应的影响。在车削（对照）钛表面以及用45微米（标准）、45 - 63微米和63 - 90微米二氧化钛颗粒喷砂处理的表面上研究了成纤维细胞的形态和附着情况。使用共聚焦激光扫描仪和扫描电子显微镜对标本进行分析。使用半自动交互式图像分析仪测量细胞轮廓面积。数据以附着百分比表示。车削样品的表面最光滑，而用63 - 90微米颗粒喷砂处理的表面最粗糙。所有经二氧化钛喷砂处理的标本表面均均匀。细胞似乎变平、铺展并与相邻细胞形成细胞桥。车削钛表面上的成纤维细胞似乎沿着表面细微不规则处的方向排列，但在喷砂处理的表面上倾向于随机铺展。附着试验表明，与车削表面相比，标准表面上成纤维细胞的附着百分比没有显著差异。用45 - 63微米或63 - 90微米颗粒喷砂处理的表面，其细胞附着百分比均显著低于对照（P < 0.05）。用63 - 90微米颗粒喷砂处理的表面细胞附着率最低。在1 - 72小时后，发现颗粒大小程度与成纤维细胞附着之间存在显著相关性（P < 0.01）。得出的结论是，表面微观纹理会影响人牙龈成纤维细胞的附着和生长：用45微米二氧化钛喷砂处理的表面不会抑制成纤维细胞的附着，光滑或有细微凹槽的表面可能有利于细胞附着。
