Fischer Nicholas G, Wong Jeffrey, Baruth Andrew, Cerutis D Roselyn
Department of Physics, College of Arts and Sciences, Creighton University, 2500 California Plaza, Omaha, NE 68178, USA.
Department of Oral Biology, School of Dentistry, Creighton University, 2802 Webster Street, Omaha, NE 68178, USA.
Materials (Basel). 2017 Nov 27;10(12):1358. doi: 10.3390/ma10121358.
Mucosal seal formation around dental abutments is critical to the successful integration of dental implants into the human oral cavity. No information exists for how clinically relevant polishing procedures for computer-aided design and computer-aided manufactured (CAD/CAM) zirconia abutments affects cellular responses important to mucosal seal formation. CAD/CAM zirconia was divided into four groups for clinically relevant polishing utilizing commercial polishing heads: control, coarse, coarse plus medium, and coarse plus medium plus fine. Surfaces were analyzed with scanning electron microscopy (SEM), atomic force microscopy (AFM), and optical profilometry (OP). Subsequently, human gingival fibroblasts (HGFs) were seeded onto the zirconia surfaces. Proliferation was measured via a quantitative SEM technique and focal adhesion kinase (FAK) phosphorylation status was measured by an enzyme-linked immunosorbent assay (ELISA). Results showed an increase in proliferation on all polished surfaces as compared to the control. Phosphorylation of FAK at tyrosine 397 (Y397) was up-modulated on the control surfaces. The associated cell adaptation is discussed. In all cases, FAK phosphorylation was greater at 24 h than 48 h. These results suggest that clinicians should be mindful of the effects of abutment polishing methodology, as this may have an impact on early mucosal seal formation.
牙基周围的黏膜封闭形成对于牙种植体成功融入人体口腔至关重要。目前尚无关于计算机辅助设计和计算机辅助制造（CAD/CAM）氧化锆牙基的临床相关抛光程序如何影响对黏膜封闭形成重要的细胞反应的信息。将CAD/CAM氧化锆分为四组，使用商用抛光头进行临床相关抛光：对照组、粗抛组、粗抛加中抛组和粗抛加中抛加细抛组。通过扫描电子显微镜（SEM）、原子力显微镜（AFM）和光学轮廓仪（OP）对表面进行分析。随后，将人牙龈成纤维细胞（HGFs）接种到氧化锆表面。通过定量SEM技术测量细胞增殖，并通过酶联免疫吸附测定（ELISA）测量粘着斑激酶（FAK）的磷酸化状态。结果显示，与对照组相比，所有抛光表面上的细胞增殖均增加。在对照表面上，酪氨酸397（Y397）处的FAK磷酸化上调。讨论了相关的细胞适应性。在所有情况下，24小时时的FAK磷酸化均高于48小时。这些结果表明，临床医生应注意牙基抛光方法的影响，因为这可能会对早期黏膜封闭形成产生影响。
