Hofling J F, Spolidori D M, Rosa E A, Pereira C V, Moreira D
Department of Oral Diagnosis, School of Dentistry, University of Campinas, Sao Paulo, Brazil.
Indian J Dent Res. 1998 Jul-Sep;9(3):91-7.
Saliva samples from students aged 6 to 8 year-old were analysed in order to determine the incidence of Streptococcus group mutans and Lactobacillus. Two hundred children were examined, distributed in five socioeconomic categories (A to E). Stimulated saliva samples were collected and inoculated into the SB20 and Rogsa agar culture medium for the Streptococcus and Lactobacillus cultivation. After growth, the number of these microorganisms (CUF/mL) was determined after identification of the representative colonies by biochemical methods on the basis of carbohydrate fermentation. A significative part of the population, particularly among the lower socioeconomic categories (D/E) was considered a high risk group in developing dental caries because of the high number of Streptococcus group mutans and Lactobacillus.
对6至8岁学生的唾液样本进行分析,以确定变形链球菌群和乳酸菌的发病率。检查了200名儿童,他们分布在五个社会经济类别(A至E)中。收集刺激后的唾液样本,并接种到SB20和罗氏琼脂培养基中,用于培养链球菌和乳酸菌。培养后,在通过基于碳水化合物发酵的生化方法鉴定代表性菌落之后,确定这些微生物的数量(CFU/mL)。由于变形链球菌群和乳酸菌数量众多,相当一部分人群,特别是社会经济地位较低的类别(D/E)被认为是患龋齿的高风险群体。