Triana O, Jaramillo N, Moreno J
Biology Department, Faculty of Exact and Natural Sciences, University of Antioquia, Medellín, Colombia.
Biol Res. 1999;32(1):1-10.
This paper presents our study of genetic variability of Trypanosoma cruzi and Trypanosoma rangeli strains isolated from different Colombian biological hosts, using multilocus enzyme electrophoresis for 15 enzyme systems and electrophoretic analysis of kinetoplast DNA (kDNA) digested with EcoRI and MspI endonucleases. Isoenzyme profiles were used to determine genotypes for each of the strains. Populations of T. cruzi and T. rangeli were found to have a polymorphic average of 86.7% and 26.7%, respectively. Schyzodeme analysis showed high variability for T. cruzi, since its genetic distance values were found to be greater than 50%, considerably higher than those previously reported for several T. cruzi strains from other countries. These results suggest that Colombian strains should be considered as genetically independent entities and are worth studying independently from each other to clearly establish their biological and clinical characteristics.
本文介绍了我们对从不同哥伦比亚生物宿主中分离出的克氏锥虫和兰氏锥虫菌株的遗传变异性的研究，采用了针对15种酶系统的多位点酶电泳以及用EcoRI和MspI内切酶消化的动基体DNA（kDNA）的电泳分析。同工酶谱用于确定每个菌株的基因型。发现克氏锥虫和兰氏锥虫群体的多态性平均值分别为86.7%和26.7%。裂殖体分析显示克氏锥虫具有高度变异性，因为其遗传距离值大于50%，大大高于先前报道的来自其他国家的几种克氏锥虫菌株。这些结果表明，哥伦比亚菌株应被视为遗传上独立的实体，值得相互独立研究，以明确确定它们的生物学和临床特征。
