Tekavcic E, Skrk J, Suhar A, Lebez D
Neoplasma. 1976;23(5):515-22.
The activity of intracellular proteinases from Ehrlich ascites carcinoma bearing mice were compared with that from liver cells of normal mice and rats. The activity of intracellular proteinases was measured in the supernatant of Ehrlich ascites carcinoma cells homogenate. The activity of intracellular proteinases in normal mouse and normal rat liver were different at pH 3.5, pH 6.0 and pH 7.5. The activity in liver cells from Ehlrich ascites carcinoma bearing mouse at pH 3.5 was not significantly changed from normal mouse or rat liver cells, however at pH 6.0 and pH 7.5 the activity in the affected liver significantly decreased. The proteolytic activity in the supernatant of Ehrlich ascites tumor cell homogenate was 0.110 E750 mmu/mgN at pH 7.5, 0.154 E750 mmu/mgN at pH 3.5. The proteolytic activity at pH 6.0 was not detected in any experiment.
将荷艾氏腹水癌小鼠细胞内蛋白酶的活性与正常小鼠和大鼠肝细胞内蛋白酶的活性进行了比较。细胞内蛋白酶的活性在艾氏腹水癌细胞匀浆的上清液中进行测定。正常小鼠和正常大鼠肝脏细胞内蛋白酶在pH 3.5、pH 6.0和pH 7.5时的活性不同。荷艾氏腹水癌小鼠肝细胞在pH 3.5时的活性与正常小鼠或大鼠肝细胞相比无显著变化，然而在pH 6.0和pH 7.5时，患病肝脏中的活性显著降低。艾氏腹水肿瘤细胞匀浆上清液在pH 7.5时的蛋白水解活性为0.110 E750毫微单位/毫克氮，在pH 3.5时为0.154 E750毫微单位/毫克氮。在任何实验中均未检测到pH 6.0时的蛋白水解活性。
