Anachkova B, Russev G
Acta Biol Med Ger. 1977;36(11-12):1945-50.
Chromatin from rat liver and Ehrlich ascites tumor (EAT) was isolated by two different procedures and the chromatin preparations were incubated at 37 degrees C. To follow the proteolysis at 0, 4, 8, and 24 hrs, aliquots were taken and analysed by SDS polyacrylamide gel electrophoresis. Although there were some differences in the proteolytic activity of the different chromatins, in general they were found to sustain a several hours incubation without appreciable degradation to occur. However, when prior to incubation the chromatins were dissociated, a rapid proteolysis took place. This shows that there are specific, chromatin bound proteinases that are inactive while immobilized in the chromatin structure and become activated on dissociation.
通过两种不同的方法分离大鼠肝脏和艾氏腹水瘤（EAT）的染色质，并将染色体制剂在37℃下孵育。为了追踪0、4、8和24小时的蛋白水解情况，取出等分试样并通过SDS聚丙烯酰胺凝胶电泳进行分析。尽管不同染色质的蛋白水解活性存在一些差异，但总体上发现它们能够承受数小时的孵育而不会发生明显降解。然而，在孵育前将染色质解离时，会发生快速的蛋白水解。这表明存在特定的、与染色质结合的蛋白酶，它们在固定于染色质结构中时无活性，而在解离时被激活。
