Gottshall R Y, Nelson E, Angela F, Anderson G
Health Lab Sci. 1975 Jan;12(1):35-40.
Storage of concentrated suspensions of Bordetella pertussis organisms at 5 C for long periods of time in the presence of 0.02% thimerosal as well as heating at 56 C for 30 minutes cannot be relied upon to detoxify the vaccines. Our data show that the toxic thimerosal-killed vaccines can be rapidly detoxified by adding formalin to a final concentration of 0.025-0.05% and incubating at temperatures of 5-20 C for from one to three months. These formalin-detoxified vaccines are protective and meet present potency requirements for pertussis vaccine. Addition of larger amounts of formalin at higher temperatures may detoxify the vaccine but may also reduce the protective effect.
百日咳博德特氏菌浓缩悬液在0.02%硫柳汞存在的情况下于5℃长期储存，以及在56℃加热30分钟，都不能确保疫苗解毒。我们的数据表明，通过添加终浓度为0.025 - 0.05%的福尔马林，并在5 - 20℃下孵育一至三个月，可迅速使含硫柳汞的有毒疫苗解毒。这些经福尔马林解毒的疫苗具有保护性，且符合目前百日咳疫苗的效力要求。在较高温度下添加大量福尔马林可能会使疫苗解毒，但也可能降低其保护效果。
