Lipid peroxidation and antioxidant state after laparoscopic and open cholecystectomy.

OBJECTIVE

To measure the amount of lipid peroxidation and erythrocyte antioxidation in patients undergoing laparoscopic and open cholecystectomy and healthy controls.

DESIGN

Non-randomised study.

SETTING

University hospital, Istanbul.

SUBJECTS

31 patients, of whom 14 underwent open and 17 laparoscopic cholecystectomy, and 15 healthy controls.

INTERVENTIONS

Heparinised blood samples were taken from the patients immediately after operation and from the healthy controls.

MAIN OUTCOME MEASURES

Lipid peroxidation index as expressed by thiobarbituric-acid-reactive substances (TBARS) and components of the erythrocyte antioxidant defence system, namely reduced glutathione, reduced glutathione peroxidase (glutathione-Px) and CuZn superoxide dismutase (CuZn SOD) in patients undergoing open or laparoscopic cholecystectomy and healthy controls.

RESULTS

All 4 variables were significantly higher in the cholecystectomy groups than in controls (p < 0.001), and laparoscopic cholecystectomy caused significantly less oxidative stress than the open operation (p < 0.001).

CONCLUSION

Both types of cholecystectomy cause oxidative stress and lead to an adaptive antioxidant response in the body. However; both oxidative stress and the antioxidant response are more pronounced after traditional open cholecystectomy.