Lee I K, Olsen S C, Kehrli M, Bolin C A
National Animal Disease Center, Agriculture Research Service, United States Department of Agriculture, Ames, Iowa 50010, USA.
Can J Vet Res. 1999 Oct;63(4):284-7.
This study was designed to determine if a single 0.5 microg administration of recombinant murine interleukin-12 (IL-12) would influence immune responses of mice vaccinated with live or killed Brucella abortus strain RB51 (SRB51). Mice were vaccinated intraperitoneally with 5 x 10(8) cfu of live or gamma-irradiated SRB51 bacteria alone, or in combination with 0.5 microg of IL-12. Control mice received saline or 0.5 microg of IL-12. Serologic responses and spleen weights after vaccination were greater in mice vaccinated with live SRB51 when compared to mice receiving killed SRB51 or control treatments. Administration of a single dose of IL-12 as a vaccine adjuvant did not influence immune responses, clearance of live SRB51, or resistance against B. abortus strain 2308 (S2308) challenge. The results of this study suggest that a single administration of 0.5 microg of IL-12 at the time of vaccination does not have significant adjuvant effects on vaccine-induced immune responses against live or killed Brucella.
本研究旨在确定单次给予0.5微克重组鼠白细胞介素-12（IL-12）是否会影响用活的或灭活的布鲁氏菌RB51株（SRB51）疫苗接种的小鼠的免疫反应。小鼠分别单独腹腔注射5×10⁸cfu活的或经γ射线照射的SRB51细菌，或与0.5微克IL-12联合注射。对照小鼠接受生理盐水或0.5微克IL-12。与接受灭活SRB51或对照处理的小鼠相比，接种活SRB51的小鼠接种后的血清学反应和脾脏重量更大。作为疫苗佐剂单次给予IL-12并不影响免疫反应、活SRB51的清除或对布鲁氏菌2308株（S2308）攻击的抵抗力。本研究结果表明，接种疫苗时单次给予0.5微克IL-12对疫苗诱导的针对活的或灭活布鲁氏菌的免疫反应没有显著的佐剂作用。
