Olsen S C
Bacteriologic Diseases Research Unit, National Animal Disease Center, Agricultural Research Service, United States Department of Agriculture, Ames, IA 50010, USA.
Res Vet Sci. 2000 Oct;69(2):135-40. doi: 10.1053/rvsc.2000.0405.
The efficacy of a reduced dosage of Brucella abortus strain RB51 (SRB51) was evaluated in adult cattle. Hereford heifers were vaccinated with saline, or three SRB51 dosing regimens (3 x 10(9) colony forming units (cfu) once, 1 x 10(9) cfu once, or 1 x 10(9) cfu twice). Cattle vaccinated with 3 x 10(9) cfu of SRB51 had greater (P < 0.05) antibody responses than non-vaccinates, and greater lymphocyte proliferative responses to SRB51 than all other treatments. Four of six non-vaccinated heifers aborted after mid-gestational challenge with B abortus strain 2308 (S2308) and S2308 was recovered from tissues obtained from all the non-vaccinates. In comparison, S2308 or SRB51 were not recovered from tissues from heifers vaccinated with any of the SRB51 dosage regimens. The data suggest that vaccination with a reduced dosage of SRB51 protects adult cattle against abortion or infection caused by exposure to virulent B abortus during the subsequent pregnancy.
在成年牛中评估了减毒布鲁氏菌RB51菌株（SRB51）的效力。给赫里福德小母牛接种生理盐水，或三种SRB51给药方案（3×10⁹菌落形成单位（cfu）一次、1×10⁹cfu一次或1×10⁹cfu两次）。接种3×10⁹cfu SRB51的牛比未接种的牛有更强（P<0.05）的抗体反应，并且对SRB51的淋巴细胞增殖反应比所有其他处理更强。六头未接种的小母牛中有四头在妊娠中期用布鲁氏菌2308菌株（S2308）攻击后流产，并且从所有未接种的牛获得的组织中分离出S2308。相比之下，在接种任何SRB51给药方案的小母牛的组织中未分离出S2308或SRB51。数据表明，用减毒剂量的SRB51进行疫苗接种可保护成年牛在随后的妊娠期间免受强毒布鲁氏菌暴露引起的流产或感染。
