Kandulska K, Szkudelski T, Nogowski L
Department of Animal Physiology and Biochemistry, University of Agriculture, Poznań, Poland.
Physiol Res. 1999;48(2):113-7.
Isolated rat adipocytes were incubated with adrenaline, adrenaline plus insulin, alloxan or alloxan plus insulin. Glycerol release was taken as a measure of lipolysis. It was observed that alloxan in the concentration of 3, 10 and 20 mmol/l intensifies lipolysis in adipocytes in the absence of adrenaline. Insulin (10(-6) mol/l) treatment of cells did not inhibit lipolysis caused by this compound, but significantly restricted lipolysis induced by adrenaline (10(-6) mol/l). It was also shown that alloxan in the concentration of 3 and 10 mmol/l intensified lipolysis stimulated by adrenaline (10(-6) mol/l). Addition of 20 mmol/l of alloxan strongly inhibited glycerol release in the presence of adrenaline. The results presented here clearly indicate that the action of alloxan concerns cells of the white adipose tissue.
将分离出的大鼠脂肪细胞与肾上腺素、肾上腺素加胰岛素、四氧嘧啶或四氧嘧啶加胰岛素一起孵育。以甘油释放量作为脂解作用的指标。观察到，在无肾上腺素的情况下，浓度为3、10和20 mmol/l的四氧嘧啶会增强脂肪细胞中的脂解作用。用胰岛素（10⁻⁶ mol/l）处理细胞并未抑制该化合物引起的脂解作用，但显著限制了由肾上腺素（10⁻⁶ mol/l）诱导的脂解作用。还表明，浓度为3和10 mmol/l的四氧嘧啶会增强由肾上腺素（10⁻⁶ mol/l）刺激的脂解作用。在存在肾上腺素的情况下，添加20 mmol/l的四氧嘧啶会强烈抑制甘油释放。此处给出的结果清楚地表明，四氧嘧啶的作用涉及白色脂肪组织的细胞。
