Ivanov V V, Shakhristova E V, Stepovaya E A, Zhavoronok T V, Novitsky V V
Department of Biochemistry and Molecular Biology, Siberian State Medical University, Federal Agency of Health Care and Social Development, Tomsk, Russia.
Bull Exp Biol Med. 2011 Jul;151(3):314-7. doi: 10.1007/s10517-011-1318-8.
In vitro experiments on cultured adipocytes from epididymal adipose tissue showed that addition of alloxan (0.5-10.0 mmol/liter) to the incubation medium induced the development of oxidative stress accompanied by an increase in the concentration of reactive oxygen species, TBA-reactive substances, and lipid hydroperoxides in cells. The redox state of adipocytes changed significantly under these conditions, which was associated with a decrease in the amount of reduced tripeptide, an increase in the content of glutathione disulfide, and a decrease in the reduced/oxidized glutathione ratio. The development of oxidative stress in adipocytes was accompanied by activation of spontaneous lipolysis, which probably plays an important role in the mechanisms of insulin resistance.
对来自附睾脂肪组织的培养脂肪细胞进行的体外实验表明，向孵育培养基中添加四氧嘧啶（0.5 - 10.0 mmol/升）会诱导氧化应激的发生，同时细胞中活性氧物质、TBA反应性物质和脂质氢过氧化物的浓度增加。在这些条件下，脂肪细胞的氧化还原状态发生了显著变化，这与还原型三肽量的减少、谷胱甘肽二硫化物含量的增加以及还原型/氧化型谷胱甘肽比率的降低有关。脂肪细胞中氧化应激的发生伴随着自发脂解的激活，这可能在胰岛素抵抗机制中起重要作用。
