Simain-Sato F, Lahmouzi J, Kalykakis G K, Heinen E, Defresne M P, De Pauw M C, Grisar T, Legros J J, Legrand R
Department of Oral Surgery, Implantology, and Periodontology, University of Liège, Belgium.
J Periodontol. 1999 Oct;70(10):1234-9. doi: 10.1902/jop.1999.70.10.1234.
The use of membranes in guided tissue regeneration (GTR) can limit the apical migration of gingival cells and favor the establishment of new attachment by periodontal ligament fibroblasts. However, gingival recession during healing following GTR has been described as a frequent complication. The purpose of this study was to determine if gingival fibroblasts are affected by the composition of the bioabsorbable membranes used in mucogingival surgery.
Two type of bioabsorbable regenerative materials were used as cell carriers. Wistar rat gingival fibroblasts (RGF) were obtained from attached gingiva, cut into small fragments, and placed in culture dishes. When confluent, cells were detached using trypsin and identified as "first transferred cells" (P1). At the third passage (P3), cell count, trypan blue exclusion test, acid phosphatase activity, DNA synthesis, phase contrast microscopy, and scanning electron microscopy were performed. The cells were then placed in wells containing the membranes and incubated for 72 hours.
When examined under microscopy, the control wells (without membranes) showed one cell type with the elongated appearance characteristic of fibroblasts. The wells with membranes showed an altered cell morphology with a high proportion of cell fragments regardless of the type of membrane used.
These results suggest that cell carrier membranes could affect RGF morphology and thus alter gingival tissue healing following GTR.
在引导组织再生（GTR）中使用的膜可以限制牙龈细胞的根尖迁移，并有利于牙周膜成纤维细胞建立新的附着。然而，GTR术后愈合过程中的牙龈退缩被描述为一种常见的并发症。本研究的目的是确定牙龈成纤维细胞是否受用于龈黏膜手术的生物可吸收膜的成分影响。
两种生物可吸收再生材料用作细胞载体。从附着龈获取Wistar大鼠牙龈成纤维细胞（RGF），切成小碎片，置于培养皿中。细胞汇合后，用胰蛋白酶消化使其脱离，鉴定为“第一代传代细胞”（P1）。在第三代传代（P3）时，进行细胞计数、台盼蓝排斥试验、酸性磷酸酶活性、DNA合成、相差显微镜检查和扫描电子显微镜检查。然后将细胞置于含有膜的孔中，孵育72小时。
在显微镜下检查时，对照孔（无膜）显示一种具有成纤维细胞典型细长外观的细胞类型。无论使用何种类型的膜，含膜的孔均显示细胞形态改变，细胞碎片比例很高。
这些结果表明，细胞载体膜可能影响RGF的形态，从而改变GTR术后牙龈组织的愈合。
