神经细胞和黏附分子对周围神经再生神经导管的影响。
Effects of nerve cells and adhesion molecules on nerve conduit for peripheral nerve regeneration.
作者信息
Chung Joo-Ryun, Choi Jong-Won, Fiorellini Joseph P, Hwang Kyung-Gyun, Park Chang-Joo
机构信息
Division of Oral and Maxillofacial Surgery, Department of Dentistry, College of Medicine, Hanyang University, Seoul, Korea.
Department of Periodontics, University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USA.
出版信息
J Dent Anesth Pain Med. 2017 Sep;17(3):191-198. doi: 10.17245/jdapm.2017.17.3.191. Epub 2017 Sep 25.
BACKGROUND
For peripheral nerve regeneration, recent attentions have been paid to the nerve conduits made by tissue-engineering technique. Three major elements of tissue-engineering are cells, molecules, and scaffolds.
METHODS
In this study, the attachments of nerve cells, including Schwann cells, on the nerve conduit and the effects of both growth factor and adhesion molecule on these attachments were investigated.
RESULTS
The attachment of rapidly-proliferating cells, C6 cells and HS683 cells, on nerve conduit was better than that of slowly-proliferating cells, PC12 cells and Schwann cells, however, the treatment of nerve growth factor improved the attachment of slowly-proliferating cells. In addition, the attachment of Schwann cells on nerve conduit coated with fibronectin was as good as that of Schwann cells treated with glial cell line-derived neurotrophic factor (GDNF).
CONCLUSIONS
Growth factor changes nerve cell morphology and affects cell cycle time. And nerve growth factor or fibronectin treatment is indispensable for Schwann cell to be used for implantation in artificial nerve conduits.
背景
对于周围神经再生，组织工程技术制备的神经导管近来受到关注。组织工程的三个主要要素是细胞、分子和支架。
方法
在本研究中，研究了包括雪旺细胞在内的神经细胞在神经导管上的附着情况，以及生长因子和黏附分子对这些附着的影响。
结果
快速增殖细胞C6细胞和HS683细胞在神经导管上的附着优于缓慢增殖细胞PC12细胞和雪旺细胞，然而，神经生长因子处理改善了缓慢增殖细胞的附着。此外，雪旺细胞在涂有纤连蛋白的神经导管上的附着与用胶质细胞源性神经营养因子（GDNF）处理的雪旺细胞的附着一样好。
结论
生长因子改变神经细胞形态并影响细胞周期时间。并且神经生长因子或纤连蛋白处理对于雪旺细胞用于人工神经导管植入是必不可少的。
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