尼古丁在人体细胞中诱导的蛋白酶活性。

Protease activity induced by nicotine in human cells.

作者信息

Wang X L, Kita K, Takahashi S, Hasegawa R, Chi X J, Yamamori H, Nakajima N, Yokoe H, Tanzawa H, Suzuki N

机构信息

Department of Oral Surgery, Chiba University School of Medicine, Chuo-ku, Chiba 260-8670, Japan.

出版信息

Int J Mol Med. 1999 Nov;4(5):537-40. doi: 10.3892/ijmm.4.5.537.

DOI:10.3892/ijmm.4.5.537

PMID:10534577

Abstract

Nicotine has a wide range of biological effects, and proteases have been extensively studied for their biological roles in living creatures. The aim of this study is to determine whether nicotine can induce proteolytic protease activity in cultures of various human cell lines. Plasminogen activator-like fibrinolytic protease activity, using 125I-fibrin as substrate in the presence of plasminogen, was estimated in cells with and without nicotine treatment. Among 16 cell lines tested, APr-1 cells were found to have the highest induced protease activity. Partial purification of the proteases was carried out by high performance liquid chromatography gel filtration on TSKG2000SW. Protease inhibitor tests indicated that the proteases induced by nicotine are serine proteases.

摘要

尼古丁具有广泛的生物学效应，并且蛋白酶因其在生物体内的生物学作用而受到广泛研究。本研究的目的是确定尼古丁是否能在各种人类细胞系培养物中诱导蛋白水解蛋白酶活性。在有和没有尼古丁处理的细胞中，以125I-纤维蛋白为底物，在纤溶酶原存在的情况下，估计类纤溶酶原激活剂的纤维蛋白溶解蛋白酶活性。在测试的16种细胞系中，发现APr-1细胞具有最高的诱导蛋白酶活性。通过在TSKG2000SW上进行高效液相色谱凝胶过滤对蛋白酶进行部分纯化。蛋白酶抑制剂试验表明，尼古丁诱导的蛋白酶是丝氨酸蛋白酶。