Ji S G, Chai Y F, Zhang G Q, Wu Y T, Liang D S, Xu Z M
Department of Pharmacy, Hospital 401, Qingdao 266071, People's Republic of China.
Biomed Chromatogr. 1999 Nov;13(7):439-41. doi: 10.1002/(SICI)1099-0801(199911)13:7<439::AID-BMC907>3.0.CO;2-U.
A non-aqueous capillary electrophoretic method was established for the determination of berberine in Rhizoma coptidis and its preparations. The effects of organic solvent and the concentrations of sodium acetate were studied, which showed that berberine in extracts of traditional Chinese medicine can be separated successfully in a buffer solution of 75 mmol/L of sodium acetate in methanol containing 1 mol/L of acetic acid. The simple and rapid method was linear in the range 25-200 microgram/mL of berberine and had a good reproducibility, with the relative standard deviation below 2%. Non-aqueous capillary electrophoresis is a satisfactory system for the analysis of alkaloids in traditional Chinese medicine.
建立了一种非水毛细管电泳法测定黄连及其制剂中的小檗碱。研究了有机溶剂和醋酸钠浓度的影响，结果表明，在含1 mol/L醋酸的75 mmol/L醋酸钠甲醇缓冲溶液中，中药提取物中的小檗碱能够成功分离。该方法简便快速，小檗碱在25 - 200微克/毫升范围内呈线性，重现性良好，相对标准偏差低于2%。非水毛细管电泳是分析中药生物碱的一种令人满意的体系。
