Dai Kai-jin, Luo Qi-zhi, Luo Jia-bo, Ma An-de
Key Laboratory of New Drug Development in Traditional Chinese Medicine, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2003 Jul;23(7):683-5.
To develop a method for alkaloid isolation from the extract of Gegenqinlian decoction (a traditional Chinese herbal preparation) and content determination.
After the preparation of the Gegenqinlian decoction extract and the micropellets, the quaternary alkaloids of Coptis chinensis were isolated by high-performance capillary electrophoresis (HPCE) in a buffer solution containing 60% sodium phosphate (60 mmol/L, pH 8.0) and 40% methanol. 2-(4-hydroxyphenyl) ethylammonium chloride was used as the internal standard and the ultraviolet detection was performed at 254 nm for determining the contents of berberine, jatrorrhizine and palmatine in the extract of Gegenqinlian decoction.
Berberine, jatrorrhizine and palmatine were successfully isolated within 6 min with recovery rates of 99.05%, 98.70% and 97.76% respectively and relative standard deviation of 2.12%, 1.85% and 1.95% respectively.
HPCE is applicable for the determination and isolation of quaternary alkaloids in Coptis chinensis with high accuracy and recovery rate.
建立从葛根芩连汤（一种传统中药制剂）提取物中分离生物碱并进行含量测定的方法。
制备葛根芩连汤提取物及微丸后，采用高效毛细管电泳（HPCE）在含60%磷酸钠（60 mmol/L，pH 8.0）和40%甲醇的缓冲溶液中分离黄连的季铵生物碱。以2-(4-羟基苯基)乙氯化铵为内标，于254 nm波长处进行紫外检测，测定葛根芩连汤提取物中小檗碱、药根碱和巴马汀的含量。
小檗碱、药根碱和巴马汀在6 min内成功分离，回收率分别为99.05%、98.70%和97.76%，相对标准偏差分别为2.12%、1.85%和1.95%。
高效毛细管电泳法适用于黄连中季铵生物碱的测定和分离，具有较高的准确度和回收率。
