Barrios H, Valderrama B, Morett E
Departamento de Reconocimiento Molecular y Bioestructura, Instituto de Biotecnología, Universidad Nacional Autónoma de México, Cuernavaca, Morelos 62271, México.
Nucleic Acids Res. 1999 Nov 15;27(22):4305-13. doi: 10.1093/nar/27.22.4305.
Promoters recognized by the RNA-polymerase with the alternative sigma factor sigma(54) (Esigma54) are unique in having conserved positions around -24 and -12 nucleotides upstream from the transcriptional start site, instead of the typical -35 and -10 boxes. Here we compile 186 -24/-12 promoter sequences reported in the literature and generate an updated and extended consensus sequence. The use of the extended consensus increases the probability of identifying genuine -24/-12 promoters. The effect of several reported mutations at the -24/-12 elements on RNA-polymerase binding and promoter strength is discussed in the light of the updated consensus.
由带有替代σ因子σ⁵⁴（Esigma54）的RNA聚合酶识别的启动子具有独特之处，即在转录起始位点上游约-24和-12个核苷酸处有保守位置，而非典型的-35和-10框。在此，我们汇编了文献中报道的186个-24/-12启动子序列，并生成了一个更新且扩展的共有序列。使用扩展的共有序列增加了识别真正-24/-12启动子的概率。根据更新后的共有序列，讨论了-24/-12元件处几个已报道的突变对RNA聚合酶结合和启动子强度的影响。
