Ikeda T, Xia X Y, Xia Y X, Ikenoue T
Department of Obstetrics and Gynecology, Miyazaki Medical College, 5200 Kihara, Kiyotake, Miyazaki, Japan.
Brain Res Dev Brain Res. 1999 Oct 20;117(1):53-8. doi: 10.1016/s0165-3806(99)00097-8.
Effect of hyperthermic preconditioning on disruption of blood-brain barrier induced by hypoxic-ischemic insult was examined. Seven-day-old Wistar rats were separated into (1) pre-heated (15 min of hyperthermia at 41.5-42.0 degrees C) or (2) non-heated group (33 degrees C). Twenty-four hours after conditioning, all rats were subjected to 2 h hypoxia-ischemia (8% oxygen/92% nitrogen, at 33 degrees C), then 24 h later all brains were examined for immunohistological staining of endogenous macromolecule, IgG extravasation and staining of microtubule-associated protein 2 (MAP2) with MAP2 loss being an early marker of neuronal damage. Significant reduction in the area of IgG staining and loss of MAP2 staining was observed in the pre-heated group as compared to that in non-heated group. There was a close relationship between IgG staining and MAP2 staining loss, with the former area always found in the latter, suggesting that extravasation associates neuronal injury. Serum cortisol concentration in pre-heated group was significantly higher than that in non-heated group 24 h after hyperthermic treatment (14.8+/-0.6 vs. 12.5+/-0.3 ng/ml, p<0.01). These data indicate that hyperthermic preconditioning prevents disruption of blood-brain barrier, resulting in amelioration of hypoxic-ischemic neuronal damage in newborn rat.
研究了热预处理对缺氧缺血性损伤诱导的血脑屏障破坏的影响。将7日龄的Wistar大鼠分为（1）预热组（在41.5 - 42.0摄氏度下进行15分钟热疗）或（2）非加热组（33摄氏度）。预处理24小时后，所有大鼠均经历2小时的缺氧缺血（8%氧气/92%氮气，33摄氏度），然后24小时后检查所有大脑，进行内源性大分子的免疫组织化学染色、IgG外渗以及微管相关蛋白2（MAP2）染色，MAP2缺失是神经元损伤的早期标志物。与非加热组相比，预热组中IgG染色面积显著减少，MAP2染色缺失。IgG染色与MAP2染色缺失之间存在密切关系，前者区域总是出现在后者区域，表明外渗与神经元损伤相关。热疗处理24小时后，预热组血清皮质醇浓度显著高于非加热组（14.8±0.6对12.5±0.3 ng/ml，p<0.01）。这些数据表明，热预处理可防止血脑屏障破坏，从而改善新生大鼠缺氧缺血性神经元损伤。
