人血清白蛋白-甲氧基聚乙二醇纳米粒的制备及其体外特性研究

Preparation and in vitro characterization of HSA-mPEG nanoparticles.

作者信息

Lin W, Garnett M C, Schacht E, Davis S S, Illum L

机构信息

Department of Pharmaceutical Sciences, University of Nottingham, University Park, Nottingham, UK.

出版信息

Int J Pharm. 1999 Nov 5;189(2):161-70. doi: 10.1016/s0378-5173(99)00253-7.

DOI:10.1016/s0378-5173(99)00253-7

PMID:10536244

Abstract

Surface modified human serum albumin (HSA) nanoparticles with a size of approximately 150 nm in diameter were prepared from a PEG-HSA conjugate, methoxy-polyethylene glycol modified human serum albumin (HSA-mPEG) using a coacervation method and crosslinked with glutaraldehyde. The zeta-potential of the surface modified nanoparticles was significantly lower than that of unmodified HSA nanoparticles. The existence of a hydrated steric barrier surrounding the nanoparticles was confirmed by electrolyte and pH induced flocculation tests. The surface modified nanoparticles showed a reduced plasma protein adsorption on the particle surface compared with unmodified particles.

摘要

采用凝聚法由聚乙二醇-人血清白蛋白共轭物（甲氧基聚乙二醇修饰的人血清白蛋白，即HSA-mPEG）制备了直径约为150 nm的表面修饰人血清白蛋白（HSA）纳米颗粒，并用戊二醛进行交联。表面修饰纳米颗粒的ζ电位显著低于未修饰的HSA纳米颗粒。通过电解质和pH诱导絮凝试验证实了纳米颗粒周围存在水合空间位垒。与未修饰颗粒相比，表面修饰纳米颗粒在颗粒表面的血浆蛋白吸附减少。