Lin W, Garnett M C, Davies M C, Bignotti F, Ferruti P, Davis S S, Illum L
Department of Pharmaceutical Sciences, University of Nottingham, UK.
Biomaterials. 1997 Apr;18(7):559-65. doi: 10.1016/s0142-9612(96)00176-7.
Surface-modified human serum albumin (HSA) nanospheres with a size of around 100 nm in diameter were prepared from poly(amidoamine)-poly(ethylene glycol) copolymer grafted human serum albumin (HSA-PAA-PEG) and poly(thioetheramido acid)-poly(ethylene glycol) copolymer grafted human serum albumin (HSA-PTAAC-PEG). The nanospheres were produced using a pH-coacervation method and cross-linked with glutaraldehyde. The cross-linking efficiency was affected by the type of albumin conjugate used. The zeta potential of the surface-modified nanospheres was significantly lower than that of unmodified particles. The existence of a hydrated steric barrier surrounding the nanospheres was confirmed by electrolyte- and pH-induced flocculation tests. The surface-modified nanospheres showed a reduced plasma protein adsorption on the particle surface compared with unmodified particles.
由聚（酰胺胺）-聚（乙二醇）共聚物接枝人血清白蛋白（HSA-PAA-PEG）和聚（硫醚酰胺酸）-聚（乙二醇）共聚物接枝人血清白蛋白（HSA-PTAAC-PEG）制备了直径约100nm的表面改性人血清白蛋白（HSA）纳米球。纳米球采用pH凝聚法制备，并用戊二醛交联。交联效率受所用白蛋白缀合物类型的影响。表面改性纳米球的zeta电位明显低于未改性颗粒。通过电解质和pH诱导的絮凝试验证实了纳米球周围存在水合空间位垒。与未改性颗粒相比，表面改性纳米球在颗粒表面的血浆蛋白吸附减少。
