Verdrengh M, Kum W, Chow A, Tarkowski A
Department of Rheumatology, University of Göteborg, Göteborg, Sweden.
Clin Exp Immunol. 1999 Nov;118(2):268-70. doi: 10.1046/j.1365-2249.1999.01061.x.
Staphylococcus aureus produces a large number of potential virulence factors, among these the superantigen toxic shock syndrome toxin-1 (TSST-1). We have recently demonstrated that TSST-1 is involved in the pathogenesis of septic arthritis. Recent data show that the TSST-1 molecule is composed of two distinct domains, one proposed to interact with T cell receptor (TCR) and one with the MHC class II. The aim of this study was to assess if interaction between TSST-1-specific MoAbs directed to sites on the MHC and/or TCR Vbeta affects the development of experimental S. aureus-induced arthritis. For that purpose we used a panel of seven MoAbs, which were injected intraperitoneally before and after inoculation with a TSST-1-producing S. aureus strain. Administration of antibodies did not affect the development of arthritis, suggesting inefficacy of such a procedure in neutralization of exotoxin-mediated disease manifestations.
金黄色葡萄球菌可产生大量潜在的毒力因子，其中包括超抗原中毒性休克综合征毒素-1（TSST-1）。我们最近证实TSST-1参与了脓毒性关节炎的发病机制。最近的数据表明，TSST-1分子由两个不同的结构域组成，一个被认为与T细胞受体（TCR）相互作用，另一个与MHC II类分子相互作用。本研究的目的是评估针对MHC和/或TCR Vβ位点的TSST-1特异性单克隆抗体之间的相互作用是否会影响实验性金黄色葡萄球菌诱导的关节炎的发展。为此，我们使用了一组七种单克隆抗体，在接种产生TSST-1的金黄色葡萄球菌菌株之前和之后腹腔注射。抗体的施用并未影响关节炎的发展，这表明该程序在中和外毒素介导的疾病表现方面无效。
