Neumann N J, Hölzle E, Wallerand M, Vierbaum S, Ruzicka T, Lehmann P
Department of Dermatology, Heinrich-Heine-University, Düsseldorf, Germany.
Photodermatol Photoimmunol Photomed. 1999 Oct;15(5):166-70. doi: 10.1111/j.1600-0781.1999.tb00078.x.
The aim of the present study was to evaluate the supposed photoprotective effects of ascorbic acid, acetylsalicylic acid, and indomethacin by the photo hen's egg test, a recently developed new model for phototoxicity. Therefore, in three independent experimental settings the blood vessel system of the embryo's yolk sac of 24 incubated hens' eggs (2 test groups) were exposed to 60 mJ/cm2 ultraviolet B (UVB) to induce severe phototoxic damage. Before UVB irradiation, one of these test groups was exposed additionally to one of the test substances and the other one to 0.9% sodium solution alone. To exclude plain toxic reactions, two additional test groups were exposed only to 0.9% sodium chloride solution or to one of the test substances alone. Over a test observation period of 24 h, the embryo lethality as well as the morphological changes of the yolk sac blood vessel system were observed. Ascorbic acid led to a significant and remarkable reduction of the UVB-induced damage. Acetylsalicylic acid also showed a significant but lower photoprotective capacity. In contrast, indomethacin showed no photoprotective effects in the photo hen's egg test.
本研究的目的是通过光鸡蛋试验评估抗坏血酸、乙酰水杨酸和吲哚美辛假定的光保护作用，光鸡蛋试验是一种最近开发的光毒性新模型。因此，在三个独立的实验设置中，将24个孵化鸡蛋（2个试验组）胚胎卵黄囊的血管系统暴露于60 mJ/cm2的紫外线B（UVB）下，以诱导严重的光毒性损伤。在UVB照射前，其中一个试验组额外暴露于一种试验物质，另一个试验组仅暴露于0.9%的钠溶液。为排除单纯的毒性反应，另外两个试验组仅暴露于0.9%的氯化钠溶液或仅暴露于一种试验物质。在24小时的试验观察期内，观察胚胎致死率以及卵黄囊血管系统的形态变化。抗坏血酸导致UVB诱导损伤显著且明显减少。乙酰水杨酸也显示出显著但较低的光保护能力。相比之下，吲哚美辛在光鸡蛋试验中未显示出光保护作用。
