Neumann N J, Hölzle E, Lehmann P, Rosenbruch M, Klaucic A, Plewig G
Department of Dermatology, Heinrich-Heine-University Duesseldorf, Germany.
Br J Dermatol. 1997 Mar;136(3):326-30.
The aim of this investigation was to establish a new model for phototoxicity which is more advanced than the widely used cultures of yeasts, bacteria or cells of various origin, and at the same time to avoid animal testing. We studied the extraembryonal vasculature of the incubated hen's egg. This model was originally introduced by toxicologists as an alternative to the rabbit's eye irritation test (Draize test). In the photo hen's egg test, substances are applied to the embryo's yolk-sac blood vessel system at a non-toxic concentration and are irradiated with 5 J/cm2 ultraviolet A (UVA) (320-400 nm). Promethazine, haematoporphyrin, ciprofloxacin and 8-methoxypsoralen were tested in this system. Death of the embryo, membrane discoloration and haemorrhage are parameters for phototoxic damage, which were recorded during an observation period of 24 h. These well-known phototoxic substances induced pronounced damage of the yolk-sac membrane and blood vessels which was not found in the controls (test substance alone, UVA alone or untreated) using a 2 x 2 factorial test design. The photo hen's egg test serves as a valid screening model for substances supposed to be photosensitizers owing to a phototoxic mechanism.
本研究的目的是建立一种比广泛使用的各种来源的酵母、细菌或细胞培养物更先进的光毒性新模型，同时避免动物试验。我们研究了孵化鸡蛋的胚外血管系统。该模型最初由毒理学家引入，作为兔眼刺激试验（Draize试验）的替代方法。在光鸡蛋试验中，将物质以无毒浓度应用于胚胎的卵黄囊血管系统，并用5 J/cm2的紫外线A（UVA）（320-400 nm）进行照射。在该系统中测试了异丙嗪、血卟啉、环丙沙星和8-甲氧基补骨脂素。胚胎死亡、膜变色和出血是光毒性损伤的参数，在24小时的观察期内进行记录。使用2×2析因试验设计，这些众所周知的光毒性物质对卵黄囊膜和血管造成了明显损伤，而在对照组（仅测试物质、仅UVA或未处理）中未发现这种损伤。光鸡蛋试验可作为一种有效的筛选模型，用于检测因光毒性机制而被认为是光敏剂的物质。
